In vitro myelosuppression and immunosuppression by ethanol.

Marked immunosuppression with minimal myelosuppression by bleomycin in vitro.

P-229: Chromosomal Analysis of Parthenogenetic Mouse Embryos Generated from In Vitro Activated Oocytes by Hydrostatic Pressure and Ethanol and Cytochalasin B

Background: Studies of preimplantation stage embryos by classic cytogenetic techniques have limitations, starting with the need for good metaphase stage when only one third of all analyzed embryos may show good quality metaphases. The incidence of chromosome anomalies in embryos produced by in vitro procedures is generally higher than that of embryos formed in vivo. Pressure specifically affect...

IMMUNOSUPPRESSION IN HUMAN UTERINE TISSUES THROUGHOUT THE REPRODUCTIVE CYCLE

The purpose of this study was to investigate and compare whether the explant supernatants prepared from endometrial tissues during the proliferative phase, secretory phase and early pregnancy differ in immunosuppressive activity. Specimens of nonpregnant endometrium from hysterectomies and normal first trimester decidua following elective aspiration termination of pregnancy were obtained. ...

P-76: Cytogenetic Investigation of Parthenogenetic Mouse Embryos Generated from In Vitro Activated Oocytes by Hydrostatic Pressure in The Presence of Calcium Ionophore and Ethanol

Background: The advances in cytogenetic techniques during the last few years have permitted not only the study of large populations of wild and domestic animals, but also the detection of chromosome anomalies in embryos. Chromosomal abnormalities are the most common cause of embryonic and fetal mortality in mammals. Most reports of chromosome anomalies in parthenogenetic embryos describe numeri...

Effect of different activators on development of activated in vitro matured caprine oocytes

This study was designed to compare the effectiveness of different activation treatments for activation of in vitro matured oocytes and their developmental potency in mCR2aa medium so as to obtain maximum number of embryos. A total of 1090 cumulus oocyte complexes (COC’s) were collected from 480 ovaries. In vitro matured oocytes were randomly divided into four groups. Group 1 in vitro matured oo...